Molecular fingerprinting of Vibrio tapetis strains using three PCR-based methods: ERIC-PCR, REP-PCR and RAPD
نویسندگان
چکیده
منابع مشابه
Parentage analysis using RAPD PCR.
DNA variation from randomly amplified polymorphic DNA (RAPD) (1, 2) has recently been helpful in addressing a wide range of evolutionary problems including the determination of paternity and maternity (3). It has been greeted with particular enthusiasm because it is fast, technically easy and requires little material (4). By using single 10 base primers of arbitrary sequences to amplify DNA wit...
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PCR amplification of repetitive bacterial DNA elements fingerprinting using the (GTG)(5) primer ((GTG)(5)-PCR) was proven to be useful for differentiation of a wide range of lactobacilli (i.e. 26 different (sub)species) at the species, subspecies and potentially up to the strain level. Using this rapid and reproducible genotypic technique, new Lactobacillus isolates recovered from different typ...
متن کاملGenetic Similarity Among the Three Egyptian Water Buffalo Flocks Using RAPD-PCR and PCR-RFLP Techniques
Genetic similarity and polymorphisms among the three Egyptian water buffalo flocks (Beheiry, Minoufy and Saidy) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Ten random primers were used to amplify DNA fragments in these three flocks. RAPD patterns with a level of polymorphism were detected among flocks. The resul...
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In the present study, Randomly Amplified Polymorphic DNA (RAPD) and Repetitive Extragenic Palindromic sequence-based Polymerase Chain Reaction (REP-PCR) were used to characterize 131 isolates of Pasteurella multocida, originating from different healthy and diseased animal species obtained from several geographical regions of Iran. The RAPD and REP-PCR generated amplified products in the range ...
متن کاملComparison of REP-, BOX-, ERIC- and (GTG)5-PCR for molecular discrimination of Salmonella serotypes
Ghent University, Faculty of Veterinary Medicine, Department of Veterinary Public Health and Food Safety, Salisburylaan 133, 9820 Merelbeke, Belgium Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium Center for Agricultural Research – Ghent, Department for animal product quality and transformation technology, Brusselsesteenweg 370, 9090 Melle, Belgium *Geertru...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2006
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao069175